Review



wnt3 proteintech  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Proteintech wnt3 proteintech
    Wnt3 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/10__1016_slash_j__isci__2026__114692-215-8-9
    Average 93 stars, based on 18 article reviews
    wnt3 proteintech - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    other:

    Article Title: Exercise increases bone mass by lactate/Gpr81 signaling pathway
    Article Snippet: S/BS), the number of osteoclasts/bone perimeter (N. OC/B.Pm), and the ES/BS 0.2–2 mm below the growth plate were analyzed using the OsteoMeasure Analysis System (Osteometrics, Decatur, GA) and ImageJ software (NIH) according to the manufacturers’ protocols.

    Incubation:

    Article Title: Expression of Wnt/ β -Catenin Signaling Pathway and Its Regulatory Role in Type I Collagen with TGF- β 1 in Scleral Fibroblasts from an Experimentally Induced Myopia Guinea Pig Model
    Article Snippet: Proteins of an equal concentration were separated on 10% SDS-PAGE and transferred to a PVDF transfer membrane (Millipore Corporation, Temecula, CA). .. The membranes were blocked in TBS containing 0.1% Tween-20 and 5% nonfat dry milk for 90 min, followed by an overnight incubation at 4°C with Wnt3 antibody (Proteintech, USA) at a 1 : 1000 dilution, β -catenin antibody (R&D, USA), and type I collagen antibody (Proteintech, USA) at a 1 : 250 dilution. .. After a rinse in TBST, the membranes were incubated for 1 h with a horseradish peroxidase-conjugated secondary antibody against rabbit or mouse IgG (Dako, Denmark) in a 1 : 5000 dilution.



    Similar Products

    wnt3  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology wnt3
    Wnt3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/Wnt-3+Antibody/pm41514503-75-10-13
    Average 94 stars, based on 1 article reviews
    wnt3 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    wnt3 proteintech  (Proteintech)
    93
    Proteintech wnt3 proteintech
    Wnt3 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/10__1016_slash_j__isci__2026__114692-215-8-9
    Average 93 stars, based on 1 article reviews
    wnt3 proteintech - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    antibodies targeting wnt3  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology antibodies targeting wnt3
    Antibodies Targeting Wnt3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/Wnt-3+Antibody/pm41414737-99-36-41
    Average 94 stars, based on 1 article reviews
    antibodies targeting wnt3 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    wnt3 antibody  (Proteintech)
    93
    Proteintech wnt3 antibody
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Wnt3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/pmc12603139-436-19-21
    Average 93 stars, based on 1 article reviews
    wnt3 antibody - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti wnt3  (Proteintech)
    93
    Proteintech anti wnt3
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Anti Wnt3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/pm40975192-90-10-11
    Average 93 stars, based on 1 article reviews
    anti wnt3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti wnt3 antibody  (Santa Cruz Biotechnology)
    94
    Santa Cruz Biotechnology anti wnt3 antibody
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Anti Wnt3 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/Wnt-3+Antibody/pm40467243-48-0-4
    Average 94 stars, based on 1 article reviews
    anti wnt3 antibody - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    wnt3  (Proteintech)
    93
    Proteintech wnt3
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Wnt3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/pmc11600767-99-15-35
    Average 93 stars, based on 1 article reviews
    wnt3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    p β catenin  (Proteintech)
    93
    Proteintech p β catenin
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    P β Catenin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/WNT3+Antibody/pmc11600767-99-12-35
    Average 93 stars, based on 1 article reviews
    p β catenin - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    anti recombinant wingless type mmtv integration site family member 3 anti wnt3 antibodies  (Servicebio Inc)
    86
    Servicebio Inc anti recombinant wingless type mmtv integration site family member 3 anti wnt3 antibodies
    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of <t>Wnt3,</t> β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Anti Recombinant Wingless Type Mmtv Integration Site Family Member 3 Anti Wnt3 Antibodies, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wnt3+antibody/actin+anti+%CE%B2/pm39084686-27-15-28
    Average 86 stars, based on 1 article reviews
    anti recombinant wingless type mmtv integration site family member 3 anti wnt3 antibodies - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of Wnt3, β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Communications Biology

    Article Title: Exercise increases bone mass by lactate/Gpr81 signaling pathway

    doi: 10.1038/s42003-025-08957-1

    Figure Lengend Snippet: A The BMM of mice from the vehicle and NG-25-treated groups were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, followed by Trap staining. Scale bars, 200 μm. B Quantifying osteoclast number and Trap-positive area in ( B ). n = 6. *** p < 0.001. Two-way ANOVA was performed followed by Dunnett’s test for multiple comparisons with experiments repeated independently three times. C RT-qPCR analysis of Trap , Nfatc1 and CTSK mRNA levels, * p < 0.05, ** p < 0.01, *** p < 0.001. n = 4. D BMM of mice from the vehicle and NG-25-treated cells were taken to induce osteoclast differentiation for 6 days with or without 10 mM lactate intervention, and Western blots analysis of p65, p-p65, TAK1 and Gpr81 proteins levels in osteoclasts. E Quantification of TAK1, Gpr81 and p-p65 protein expression levels. * p < 0.05, ** p < 0.01. n = 6. F Lactate promotes osteogenic differentiation and mineralization, and BMSC differentiation and mineralization are inhibited in iCRT(Wnt inhibitor) treated mice. The BMSC were differentiated for 7, 14 and 21 days, respectively, and then stained for ALP, von Kossa’s and Alizarine Red in the presence or absence of 10 mM lactate. G BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactate intervention, respectively, and RT-qPCR analysis of Runx2 , ALP , and Osterix mRNA levels in osteoblasts. All data are presented as mean ± SD. n = 6. ** p < 0.01., *** p < 0.001. H BMSC of mice from the vehicle and iCRT3-treated groups were taken to induce osteogenic differentiation for 14 days with or without 10 mM lactic acid intervention, and Western blots analysis of Wnt3, β-catenin, and OCN proteins levels in osteoblasts. I Quantification of Wnt3, β-catenin and OCN protein expression levels. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: The membranes were blocked with BSA and incubated with specific antibodies, including an osteocalcin antibody (Bioss, bs-0470R, 1:1000) and Wnt3 antibody (Proteintech, 28156-1-A, 1:1000).

    Techniques: Staining, Quantitative RT-PCR, Western Blot, Expressing